Which of the following shows that A is the Aunt of E?

Let's go option by option:

1) Option 1 says A is the brother of B and this makes A a male. So he cannot be E's aunt.

2) Option 2 says A is the sister of B, who is the father of C, who is the sister of D, who is the brother of E.

This makes A aunt of E.

3)Option 3 says A is the father of B which makes him a male. Thus he cannot be the aunt of E.

4) Option 4 says A is the mother of B, is the brother of C, who is the sister of D, who is the father of E.

This makes A the grandmother of E.