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Question 70

Match the LIST-I with LIST-II

List-I
Amino acid
List-II
Positive reaction/Test for functional group present in side chain of amino acid
A.GlutamineI.Hinsberg's test
B.LysineII.Neutral FeCl$$_3$$ test
C.TyrosineIII.Ceric ammonium nitrate test
D.SerineIV.Hoffman bromamide degradation


Choose the correct answer from the options given below:

A. Glutamine $$\longrightarrow\ $$ IV. 

Hoffmann bromamide degradation: Glutamine have an aliphatic primary amide group $$CONH_2$$ in its side chain. Primary amides undergoes the Hoffmann bromamide reaction when treated with $$Br_2$$ and an alkali so that it can degrade into an amine.

B. Lysine $$\longrightarrow\ $$ I. 

Hinsberg's test: Lysine contains a primary aliphatic amino group $$NH_2$$ at the terminal end $$epsilon-$$Position of its side chain. The Hinsberg's Test (using benzene sulfonyl chloride) is a test used to identify and differentiate primary amines.

C. Tyrosine $$\longrightarrow\ $$  II. 

Neutral $$FeCl_3$$ test: Tyrosine contains a phenolic group $$OH$$ attached to a benzene ring in its side chain. Phenols react characteristically with neutral ferric chloride$$FeCl_3$$ to form highly colored coordination complexes (typically violet/purple).

D. Serine $$\longrightarrow\ $$ III. 

Ceric ammonium nitrate (CAN) test: Serine have an aliphatic primary alcohol group $$CH_2OH$$ in its side chain. The Ceric Ammonium Nitrate (CAN) test is a standard functional group test used to confirm the presence of aliphatic alcohols (producing a red/pink complex).

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