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Match the LIST-I with LIST-II
| List-I Amino acid | List-II Positive reaction/Test for functional group present in side chain of amino acid | ||
|---|---|---|---|
| A. | Glutamine | I. | Hinsberg's test |
| B. | Lysine | II. | Neutral FeCl$$_3$$ test |
| C. | Tyrosine | III. | Ceric ammonium nitrate test |
| D. | Serine | IV. | Hoffman bromamide degradation |
Choose the correct answer from the options given below:
A. Glutamine $$\longrightarrow\ $$ IV.
Hoffmann bromamide degradation: Glutamine have an aliphatic primary amide group $$CONH_2$$ in its side chain. Primary amides undergoes the Hoffmann bromamide reaction when treated with $$Br_2$$ and an alkali so that it can degrade into an amine.
B. Lysine $$\longrightarrow\ $$ I.
Hinsberg's test: Lysine contains a primary aliphatic amino group $$NH_2$$ at the terminal end $$epsilon-$$Position of its side chain. The Hinsberg's Test (using benzene sulfonyl chloride) is a test used to identify and differentiate primary amines.
C. Tyrosine $$\longrightarrow\ $$ II.
Neutral $$FeCl_3$$ test: Tyrosine contains a phenolic group $$OH$$ attached to a benzene ring in its side chain. Phenols react characteristically with neutral ferric chloride$$FeCl_3$$ to form highly colored coordination complexes (typically violet/purple).
D. Serine $$\longrightarrow\ $$ III.
Ceric ammonium nitrate (CAN) test: Serine have an aliphatic primary alcohol group $$CH_2OH$$ in its side chain. The Ceric Ammonium Nitrate (CAN) test is a standard functional group test used to confirm the presence of aliphatic alcohols (producing a red/pink complex).
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