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Question 60

The presence or absence of hydroxyl group on which carbon atom of sugar differentiates RNA and DNA?

Solution

Nucleic acids are made of repeating nucleotides. Each nucleotide consists of a pentose sugar, a nitrogenous base and a phosphate group.

1. In DNA the pentose sugar is 2-deoxyribose. In RNA the sugar is ribose.
2. The two sugars differ only at one carbon atom of the ring:

• Numbering of the pentose carbon atoms follows the convention $$1', 2', 3', 4', 5'$$ (or simply 1, 2, 3, 4, 5).
• In ribose (RNA) the $$2'$$ carbon carries an -OH (hydroxyl) group.
• In 2-deoxyribose (DNA) the $$2'$$ carbon lacks this -OH; it has -H instead.

Thus, the presence (in ribose) or absence (in deoxyribose) of the hydroxyl group on the $$2^{\mathrm{nd}}$$ carbon distinguishes RNA from DNA.

Option A which is: $$2^{\mathrm{nd}}$$

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